Fereshteh Javadian; Zahra Sepehri; Mansour Amraee; Zohreh Kiani; Maryam Shahraki Mojahed; Zahra Shahi; Sepideh Pourghasemi Fetideh
Volume 22, Issue 4 , September and October 2015, , Pages 565-570
Abstract
Background and Purpose: The present study was carried out to determine the potential antibacterial agent of ethanolic extract of Hibiscus Sabdariffa against Klebsiella Pneumonia isolation of urinary tract infections. Methods and Materials:The leaf H. Sabdariffa was properly dried and pulverized ...
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Background and Purpose: The present study was carried out to determine the potential antibacterial agent of ethanolic extract of Hibiscus Sabdariffa against Klebsiella Pneumonia isolation of urinary tract infections. Methods and Materials:The leaf H. Sabdariffa was properly dried and pulverized into a coarse powder as described by Hanafy. Twenty grams of grinded powders from each plant were soaked in 60 ml of ethanol (95 %v/v) plus water, separately for one day with occasionally shaking. After one day of dissolving, materials were filtered through a Whatman no. 1 filter paper .Then the filtrates were evaporated using rotary evaporator. At last, 0.97 g of dried extract was obtained and then stored at40C in an air tight screw-cap tube. Isolation of bacteria : All 12 K. Pneumoniae were isolated from urine culture of hospitalized patients (Amir Al-Momenin Hospital, Zabol, south-eastern Iran), whom suffered from urinary tract infections during the years 2010- 2011. Isolated bacteria were identified by Gram's stain and standard biochemical tests. Sensitive strains to several antibiotics were evaluated by the disk diffusion method with standard Kirby - Bauer. Results: Results showed Klebsiella Pneumoniae isolates resistant to the antibiotics Erythromycin (75%) , Cefixime (3/58%) and Ceftazidime (3/33%). The results showed that the ethanol extract of Hibiscus tea with 10 ml / mg and l5 mg/m density have maximum inhibitory against Klebsiella Pneumonia samples and only one strain of K. Pneumoniae is inhibited in concentration 1/25. Discussion: It seems that H.Sabdariffa extracts could inhibit the growth of all of the mentioned bacteria. We noticed that the bactericidal effect of H.Sabdariffa extract was less than its bacteriostatic effects.
Ziba Foladvand; Zohreh Kiani; Fereshteh Javadian; Mehran Hesaraki; Aliakbar Nasiri; Zahra Sepehri
Volume 21, Issue 6 , January and February 2015, , Pages 1019-1027
Abstract
Background and Purpose: Development of antibiotic resistance among pathogenic bacteria motivates attempts to search for newer antimicrobial agents. In this research study, antibacterial effect of plant extract of M. communis , z.multiflora and Allium sativum on biofilm formation by strains of Staphylococcus ...
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Background and Purpose: Development of antibiotic resistance among pathogenic bacteria motivates attempts to search for newer antimicrobial agents. In this research study, antibacterial effect of plant extract of M. communis , z.multiflora and Allium sativum on biofilm formation by strains of Staphylococcus aureus resistant to the antibiotic were investigated.
Methods and Materials: 17 samples were separated from nose and throat of hospital and non-hospital personnels. The effect of extracts on biofilm formation by S.aureus and its resistant respect to the antibiotic were determined using a microplate 96 .
Results: Effect of antibacterial extracts used at different concentrations showed that, despite the relative resistance of most strains at concentrations used, the maximum sensitivity was at concentrations of 1.25, 2.5 and 5 mg/ml of extracts and concentration of 10 mg/ml of the extract was considered as MBC. Bacterial growth was observed only at 24 hours and no biofilm was observed at any of the extracts.
Conclusion : Results showed that plant extracts can reduce biofilm formation by Staphylococcus aureus and, therefore, they can play an important role in reducing disease.